My Lab Meeting

Here's a picture of one of my PowerPoint slides from today's lab presentation.
Here’s a picture of one of my PowerPoint slides from today’s lab presentation.

I presented the progress on my research at lab meeting today.

I haven’t gotten as far as I wanted to since my last lab meeting, but I did get as far as I expected to.

By the end of the summer I was expecting to have 5 of the 6 constructs successfully injected and integrated into C. elegans strains.

I’ve gotten 4 out of the 6 and the summer isn’t over yet. I’m on track to finish.

Otherwise, I’ve had a problem with the worms not staining like the ones of the person who used to work on this project.

His worms stained extremely dark. There’s so much blue color that the picture of them looks black.

My worms don’t look like that, but we aren’t sure why.

I’m going to tweak some things to try and make my strains look that way.

If the tweaks don’t work… I’ll have to check using a more complicated method to ensure that I injected the worms correctly.

That’s the gist of what I presented at the meeting.

The meeting went pretty well.

Today’s meeting was between my lab and the lab adjacent to us.

Our lab meetings are always joint meetings. The neighboring lab works on similar stuff. Both of us are small labs as well so it makes a lot of sense for us to meet together.

The professor from the other lab always asks the presenter tough questions.

I felt like I fielded everyone of his questions really well.

I felt prepared, I had answers to most of his questions.

I’m going to look for answers to the questions I don’t know in research papers.

I haven’t gotten around to describing more about what I do in lab, which might have made this post a little confusing.

I’ll try and upload a post that goes in more depth for my lab meeting presentation in the future.

That’s all for tonight.

-Mister Ed

Good and Bad

I had a strange amount of ups and downs today.

I started off by trying to install some child locks on our bathroom cabinets. Our new cat has been trying to sneak in and we think it’d be best if she doesn’t have access to all the toilet paper at once or all the chemicals that could hurt her under the sink.

Child locks come in two parts, the part you have to push to open it and the part the pushy part inserts into to keep the door locks. I’d glued the holdy part onto the cabinets on Monday.

I checked the glue today and was a bit rough in handling one of the holdy parts. It popped off in my hand. I sighed and reglued it on.

I glued on the pushy part and found it was very difficult to align it correctly with the holdy part. Fearing that I did it wrong, I only glued in one of the pushy parts today to see how it looks tomorrow.

After that frustration I went out to fix the flat in my bike. The flat had happened on Friday and I got new tubes on Monday.

I put the first tube in and it immediately popped. Figuring they can occasionally be duds, I pulled it out and put a second one in. It seemed fine and I biked off to work. It popped just before I got onto campus. I sighed and walked the rest of the way to my job.

I figured I was too pissed off to immediately get to work at lab, so I decided to make a quick appointment with an optometrist in town.

The optometrist informed me that I needed to know the exact amount of coverage my health insurance gave me for vision before I could get an appointment. On to calling the health insurance company then!

I called my health insurance and was put on hold (typical). I waited and zoned out not listening to the recorded message. Then the message said, “Goodbye,” and it hung up. I have no clue what led up to it saying goodbye, but I’m pretty sure it wasn’t the usual hold music.

I called again and avoided the previous menu options that had resulted in the robot disconnecting me. I ended up waiting an hour on hold before I got to talk to a human person.

The whole time it faked me out by switching up which robot voices were telling me about the health insurance website. Each time the voice changed I thought I had finally reached a real person only to have my hopes destroyed.

I eventually got the health insurance and the optometrist appointment set up.

After that my day improved. I stained some worms to see if they turn blue over the next two days. I checked some injected worms to see if the DNA I injected into them had successfully integrated into their genomes. IT HAD! WOOHOO!

I called my wife to come pick up my bike during the afternoon so I wouldn’t have to walk it home. She came by and put it in the car and reminded me why I love her.

At the end of the day my wife came home with two cupcakes for me.

A good day in the end despite the rocky start! 🙂

-Mister Ed